Includes bibliographical references (p. 201) and index.

Acknowledgment v
Foreword xi
Abbreviations xiii
Introduction
1 (6)
Terminology
2 (1)
Design and development of microarrays
3 (4)
Applications
7 (8)
Scientific problems
7 (3)
The pharmaceutical goal
10 (2)
The demands of biotechnical companies
12 (3)
Technology
15 (90)
Protein microarrays
15 (42)
Gene and protein expression
15 (9)
Protein sundries: from molecule to biochip
24 (18)
Protein chips
42 (15)
Nucleic and microarrays
57 (19)
Hybridization probes
57 (6)
Minimal quantities of RNA-7; What to do?
63 (7)
Production of DNA microarrays
70 (6)
Microarray detection
76 (18)
Light and radiation
76 (2)
Luminescent fluorophore T
78 (6)
High-energy laser light
84 (2)
Microarray scanner: Mode of operation
86 (4)
Operating mode of microarray imagers
90 (2)
Dynamic range
92 (1)
Pixels and spots
93 (1)
Microarray marking systems
94 (11)
Tyramide-signal amplification
94 (1)
Dendrimers....
95 (1)
Quantum dots
96 (3)
Chemiluminescence
99 (1)
Radioactivity
100 (1)
Time-resolved fluorescence
100 (5)
Instrumentation and Software
105 (36)
Microarray spotters
106 (9)
Mechanical contact spotting
110 (1)
Piezo-dispenser technology
111 (4)
Grids and spots
115 (1)
Digitizing: The microarray scanner
115 (2)
The microarray imager
116 (1)
The microarray scanner
117 (1)
Microarray software and documentation
117 (19)
Documentation of microarray experiments
119 (1)
Design of the experiment
120 (1)
Validation of the results
121 (2)
Microarray software
123 (6)
Comparison of expression data
129 (2)
Advanced analysis
131 (5)
Additional laboratory equipment and Laboratory requirements for microarray applications
136 (1)
Supplies
136 (1)
Additional helpful laboratory equipment
136 (1)
Clean-room technology
137 (4)
Cleanliness classes
137 (1)
Clean-room systems
138 (3)
Execution of the Microarray Experiment
141 (14)
Isolation and preparation of nucleic acids
141 (8)
Isolation of RNA ......
141 (1)
mRNA isolation from eukaryotes
142 (1)
mRNA isolation from bacteria
142 (1)
Specimen fabrication with the aid of cDNA synthesis
143 (1)
Hybridization on the DNA biochip
144 (1)
Preamplification with T7-RNA polymerase
144 (2)
Fabrication of the microarray samples
146 (1)
Quantification, inspection, reliability
147 (1)
Hybridization of the samples
147 (2)
Poly (L-lysine) coating
149 (1)
Protein array protocols
149 (6)
Protein array buffers
150 (2)
Verification with antibodies
152 (1)
Protein kinase array: Verification with 33P
153 (2)
High-Throughput Screening
155 (14)
Laboratory automation
156 (8)
Laboratory automation software
158 (2)
Preparation of samples
160 (1)
Analysis and reporting
160 (4)
Drug-target screening
164 (1)
Clinical chemistry and genetic screening
164 (5)
Database Research and Patents
169 (16)
Database research
169 (1)
Patents
170 (13)
Patent research
171 (6)
Patent-protected technologies
177 (6)
Brands and trademarks
183 (2)
Prospects for the Future
185 (6)
Technical developments
185 (1)
Requirement profiles
186 (3)
The end of the end
189 (2)
Appendix
191 (6)
Common buffers
191 (1)
Spotting- and washing buffers
191 (1)
Block solutions
191 (1)
Hybridization solutions
192 (1)
Formula for the calculation of the annealing temperature of oligonucleotides
192 (1)
Formula for oligonucleotides up to 15 bases
192 (1)
Formula for oligonucleotides from 20 to 70 bases
192 (1)
Molar specifications and calculations
192 (2)
Definition of a mole
192 (1)
Definition of molar mass and molarity
192 (1)
Picomolar quantities for nucleic acids
193 (1)
Definition of the dalton unit
193 (1)
Picomolar quantities for proteins and peptides
194 (1)
Calculation of the microarray spot surface area
194 (1)
Calculation of the microarray spot density
195 (1)
Spot density based on center-to-center distance
195 (1)
Spot density based on the number of absolute spots per cm2
195 (1)
Calculation of fluorophore or target density
195 (2)
Company Register 197 (4)
Web Links 201 (2)
Index 203

Microarray technology allows us to answer many questions about gene expression and drug-target screening by employing high-throughput screening. This book dedicates itself to microarrays with clear and understandable explanations and an overview of the presently available hardware, biochips and software. Separate chapters cover the different requirements for DNA and protein chips as well as spotters and scanners