000			04188cam a22003857a 4500
999			_c10771 _d10771
001			17143470
003			GZU
005			20221013122051.0
008			120131s2012 enka b 001 0 eng d
010			_a 2012359021
015			_aGBB1B0023 _2bnb
015			_aGBB1B0023 _2dnb
016	7		_a015894350 _2Uk
020			_a9780123855442 (pbk.)
020			_a0123855446 (pbk.)
022			_a9780123855442
035			_a(OCoLC)ocn754167841
040			_aUKMGB _cUKMGB _dDEBBG _dYDXCP _dCDX _dCUV _dDLC
042			_alccopycat
050	0	0	_aQH506 _b.CAR 2012
100	1		_aCarson, Sue _q(Susan) _eauthor
245	1	0	_aMolecular biology techniques : _ba classroom laboratory manual / _cby Susan Carson, Heather Miller, D. Scott Witherow.
250			_aThird edition.
260			_aOxford ; _aWaltham, MA : _bAcademic, _c2012.
300			_axxvi, 200 pages : _billustrations ; _c29 cm.
500			_aRev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006
504			_aIncludes bibliographical references and index.
505			_aLab Session 1 Getting Oriented: Practicing with Micropipettes Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation Lab Session 4 Preparation of Insert DNA (egfp) PCR Product Lab Session 5 DNA Ligation and Transformation of Escherichia coli Lab Session 6 Colony Hybridization Lab Session 7 Characterization of Recombinant Clones: Part 1 Lab Session 8 Characterization of Recombinant Clones: Part 2 Lab Session 9 Characterization of Recombinant Clones: Part 3 Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column Lab Session 14 Analysis of Purification Fractions Lab Session 15 Total RNA Purification Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 Appendix 1 Equipment Appendix 2 Prep List Appendix 3 Preparation of Competent E. coli Cells Appendix 4 Pre-Lab Questions
520			_aThis manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
650		0	_aMolecular biology _vLaboratory manuals.
700	1		_aMiller, Heather B. _eauthor
700	1		_aWitherow, D. Scott. _eauthor
906			_a7 _bcbc _ccopycat _d3 _encip _f20 _gy-gencatlg
942			_2lcc _cBK _eThird edition _h506 _iCAR _kQH