MARC details
| 000 -LEADER |
|---|
| fixed length control field |
04188cam a22003857a 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
17143470 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
GZU |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20221013122051.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
120131s2012 enka b 001 0 eng d |
| 010 ## - LIBRARY OF CONGRESS CONTROL NUMBER |
|---|
| LC control number |
2012359021 |
| 015 ## - NATIONAL BIBLIOGRAPHY NUMBER |
|---|
| National bibliography number |
GBB1B0023 |
| Source |
bnb |
| 015 ## - NATIONAL BIBLIOGRAPHY NUMBER |
|---|
| National bibliography number |
GBB1B0023 |
| Source |
dnb |
| 016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER |
|---|
| Record control number |
015894350 |
| Source |
Uk |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9780123855442 (pbk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
0123855446 (pbk.) |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
9780123855442 |
| 035 ## - SYSTEM CONTROL NUMBER |
|---|
| System control number |
(OCoLC)ocn754167841 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
UKMGB |
| Transcribing agency |
UKMGB |
| Modifying agency |
DEBBG |
| -- |
YDXCP |
| -- |
CDX |
| -- |
CUV |
| -- |
DLC |
| 042 ## - AUTHENTICATION CODE |
|---|
| Authentication code |
lccopycat |
| 050 00 - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
QH506 |
| Item number |
.CAR 2012 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Carson, Sue |
| Fuller form of name |
(Susan) |
| Relator term |
author |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Molecular biology techniques : |
| Remainder of title |
a classroom laboratory manual / |
| Statement of responsibility, etc. |
by Susan Carson, Heather Miller, D. Scott Witherow. |
| 250 ## - EDITION STATEMENT |
|---|
| Edition statement |
Third edition. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Oxford ; |
| -- |
Waltham, MA : |
| Name of publisher, distributor, etc. |
Academic, |
| Date of publication, distribution, etc. |
2012. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xxvi, 200 pages : |
| Other physical details |
illustrations ; |
| Dimensions |
29 cm. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references and index. |
| 505 ## - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Lab Session 1 Getting Oriented: Practicing with Micropipettes<br/>Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA<br/>Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation<br/>Lab Session 4 Preparation of Insert DNA (egfp) PCR Product<br/>Lab Session 5 DNA Ligation and Transformation of Escherichia coli<br/>Lab Session 6 Colony Hybridization<br/>Lab Session 7 Characterization of Recombinant Clones: Part 1<br/>Lab Session 8 Characterization of Recombinant Clones: Part 2<br/>Lab Session 9 Characterization of Recombinant Clones: Part 3<br/>Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2<br/>Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1<br/>Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2<br/>Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column<br/>Lab Session 14 Analysis of Purification Fractions<br/>Lab Session 15 Total RNA Purification<br/>Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1<br/>Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2<br/>Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1<br/>Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2<br/>Appendix 1 Equipment<br/>Appendix 2 Prep List<br/>Appendix 3 Preparation of Competent E. coli Cells<br/>Appendix 4 Pre-Lab Questions |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Molecular biology |
| Form subdivision |
Laboratory manuals. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Miller, Heather B. |
| Relator term |
author |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Witherow, D. Scott. |
| Relator term |
author |
| 906 ## - LOCAL DATA ELEMENT F, LDF (RLIN) |
|---|
| a |
7 |
| b |
cbc |
| c |
copycat |
| d |
3 |
| e |
ncip |
| f |
20 |
| g |
y-gencatlg |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Books |
| Edition |
Third edition |
| Classification part |
506 |
| Item part |
CAR |
| Call number prefix |
QH |
